Colonel Shuffle is a character in the Looney Tunes stable.
Based in the Southern United States, Colonel Shuffle has been shown as fiercely loyal to this region and deeply offended by anything that he feels reminds him of the North.
He refers to himself specifically by name in "Mississippi Hare" (1949), following a game of poker in which he lost (three queens to four kings) and proceeds to let off a barrage of gunfire. Sometimes he is shown playing a banjo in classic Dixieland style.
Shuffle played a prison warden in the Tiny Toon Adventures episode Gang Busters.