The Warners' 65th Anniversary Special is the season finale of the first season of Animaniacs.
A "live" special that highlights the anniversary of the Warners' creation, from their original roles as sidekicks to the early Looney Tunes character Buddy through their golden age and their occasional breakouts prior to recent times. Behind the scenes, though, a mysterious adversary is plotting their demise.
- Viza Vizzelli is a parody of the famous singer "Liza Minnelli"
- Buddy is a real Warner Bros. animation character who was created to replace Bosko after Harman and Ising left the studio and took the character with them. Apparently, Warner Bros. was not shy to admit the failure of the character. However Buddy was created in 1933, not 1929 (which was when the first Bosko cartoon was originally produced).
- All celebrity caricatures who were interviewed are identified under the characters they made famous in their movies. Spartacus is a caricature of Kirk Douglas, Prof. Kingsfield is a caricature of John Houseman, the Gipper is Ronald Reagan, George Bailey is Jimmy Stewart.
- Bugs Bunny, Foghorn Leghorn, Tweety, Porky Pig, Daffy Duck and Yosemite Sam made cameos in the show, and each of them would tell about the Warners' reckless behavior.
- The Warners' appearance on A Really Big Shew is a parody of the Beatles' first appearance on The Ed Sullivan Show. The song they sing, "Make a Gookie", is a parody of Simon & Garfunkel's "The 59th Street Bridge Song (Feelin' Groovy)".
- Bob Hope calls Dot "Dottie", but Dot does not say, "Call me 'Dottie' and you die" or act upon that threat. This could be because she was asleep when the camera panned over to the Warners.
- Parts of the opening and closing song for this episode would be used (set to different clips of the Warner siblings) as the introduction to Cartoon Network's Animaniacs marathon celebrating the show having joined the channel's line-up.